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KMID : 0894520080120010067
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Development & Reproduction
2008 Volume.12 No. 1 p.67 ~ p.76
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Effects of Antioxidants Treatment on the Cryopreservation of Human Hematopoietic Stem Cells
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Kim Eung-Bae
Hong Soon-Gab
Do Byung-Rok
Kim Kyung-Suk
Lee Jun-Young
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Abstract
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Oxidative damage resulted from reactive oxygen species (ROS) is one of the main causes for the decrease of the viability during in vitro culture and cryopreservation process. This experiment was performed to determine the effects of antioxidants on the human hematopoietic stem cell (HSC) during cryopreservation procedure. HSCs cultured in vitro with or without antioxidants were frozen and then examined for stem cell potential after thawing. The cell viability of thawed HSC was increased in α-tocopherol and ascorbic acid treatment group compared to control group (62.7±8.0%) and it was higher in 150 uM α-tocopherol treatment group (70.5±7.0%). No significant difference was observed in the membrane integrity in all groups. In auto-differentiation rate, no significant difference was appeared in all groups, but was lower in 150 uM α-tocopherol (7.3±2.6%) compared to control group (10.1±1.6%). These results demonstrate that treatment of antioxidants improves the efficiency of cryopreservation for HSC and α-tocopherol may be considered effective antioxidant for the protective effect on HSC.
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KEYWORD
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HSC, Antioxidant, ROS, Ascorbic acid, α-tocopherol, Viability
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